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Product Name: | Bicine | Synonyms: | n,n-dihydroxyethyl-glycin;Bicine
N,N-Bis(2-hydroxyethyl)glycine
Diethanolglycine;N,N-DI(2-HYDROXYETHYL)GLYCINE;N,N-BIS(2-HYDROXYETHYL)GLYCINE;N N-DI(HYDROXYETHYL)-B-AMINOACETIC ACID;BICINE[N,N-Di(2-hydroxyethyl)glycine];BICIN (N,N-Bis-(2-hydroxyethyl)-glycin);N,N-Di(2-hydroxyethyl)glycine | CAS: | 150-25-4 | MF: | C6H13NO4 | MW: | 163.17 | EINECS: | 205-755-1 | Product Categories: | Biochemistry;Good's Buffers;Buffer | Mol File: | 150-25-4.mol | |
| Bicine Chemical Properties |
Melting point | 190 °C (dec.)(lit.) | Boiling point | 290.25°C (rough estimate) | density | 1.05 g/mL at 20 °C | vapor pressure | 0.002-0.007Pa at 20-25℃ | refractive index | 1.4240 (estimate) | storage temp. | room temp | solubility | H2O: 1 M at 20 °C, clear, colorless | form | Powder | color | White | PH | 4.0-5.5 (25℃, 1M in H2O) | pka | 8.3(at 25℃) | PH Range | 7.6 - 9.0 | Water Solubility | soluble | λmax | λ: 260 nm Amax: 0.070 λ: 280 nm Amax: 0.040 | Merck | 14,1201 | BRN | 1769362 | LogP | 2.3 at 20℃ and pH7 | Surface tension | 72.2mN/m at 1g/L and 20℃ | CAS DataBase Reference | 150-25-4(CAS DataBase Reference) | EPA Substance Registry System | Bicine (150-25-4) |
Hazard Codes | Xn | Risk Statements | 20/21/22-36/37/38 | Safety Statements | 24/25-36-26 | WGK Germany | 3 | RTECS | MB9700000 | F | 3-10 | TSCA | Yes | HS Code | 29225000 | Toxicity | mouse,LD50,intraperitoneal,1540mg/kg (1540mg/kg),KIDNEY, URETER, AND BLADDER: "CHANGES IN TUBULES (INCLUDING ACUTE RENAL FAILURE, ACUTE TUBULAR NECROSIS)",Revue d'Epidemiologie, Medecine Sociale et Sante Publique. Vol. 10, Pg. 391, 1962. |
| Bicine Usage And Synthesis |
Chemical Properties | White/clear crystalline powder | Uses | To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM. | Uses | Biological buffer and chelating agent. | Uses | A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described. | Definition | ChEBI: N,N-bis(2-hydroxyethyl)glycine is a bicine that is a Good's buffer substance, pKa = 8.35 at 20 ℃. It is a conjugate acid of a [bis(2-hydroxyethyl)amino]acetate. It is a tautomer of a [bis(2-hydroxyethyl)ammonio]acetate. | Purification Methods | Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals — Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.] |
| Bicine Preparation Products And Raw materials |
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