| Description | 4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease. Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. |
| Chemical Properties | White Powder |
| Uses | A fluorogenic substrate in the assay of ?-glucosidase.
Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15 |
| Uses | A fluorogenic substrate in the assay of ?-glucosidase.Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15 |
| Uses | 4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:
- in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
- in β-glucosidase assay during yeast fermentation
- to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)
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| Definition | ChEBI: A beta-D-glucoside having a 4-methylumbelliferyl substituent at the anomeric position. |
| Biochem/physiol Actions | 4-Methylumbelliferyl β-D-glucopyranoside is a synthetic enzymatic substrate for glycosidase. It has been used as substrate for the β- glucosidase from enterococci. |
| Purification Methods | 4-Methylumbellifer-7-yl--Dglucopyranoside crystallises as the half hydrate from hot H2O. [Constantzas & Kocourek Collect Czech Chem Commun 24 1099 1959, De Re et al. Ann Chim (Rome) 4 9 2089 1959, Courtin-Duchateau & Veyrière Carbohydr Research 65 23, 29 1978, Beilstein 18 III/IV 5152, 8 IV 433, 18/7 V 616.] |
