Bleomycin hydrochloride

Bleomycin hydrochloride Basic information
Product Name:Bleomycin hydrochloride
Synonyms:bleomycinchlorhydrate;BleomycinA5/Pingyangmycin;BleomycinHCl;BLEOMYCIN HYDROCHLORIDES;BleoMycin A4A5 Hydrochloride;BLEOMYCIN HYDROCHLORIDE;Bleomycin hydrochloride USP/EP/BP;Bleomycin hydrochlorideQ: What is Bleomycin hydrochloride Q: What is the CAS Number of Bleomycin hydrochloride Q: What is the storage condition of Bleomycin hydrochloride
CAS:67763-87-5
MF:C50H71N16O21S2R.x(HCl)
MW:0
EINECS:
Product Categories:
Mol File:Mol File
Bleomycin hydrochloride Structure
Bleomycin hydrochloride Chemical Properties
storage temp. 4°C, protect from light
solubility DMSO: 125 mg/mL (86.15 mM)
Safety Information
MSDS Information
Bleomycin hydrochloride Usage And Synthesis
OriginatorBleomycin Hydrochloride,Nippon Kayaku, Co.,Japan
Manufacturing ProcessTo a medium having a composition of 6.4 % of millet jelly, 0.5 % of glucose, 3.5 % of soybean powder, 0.75 % of corn steep liquor, 0.3 % of sodium chloride, 0.1 % of potassium secondary phosphate, 0.05 % of zinc sulfate, 0.01 % of copper sulfate, 0.2 % of sodium nitrate and 0.01 % of Toho No. 1 (trade name for a surface active agent composed of polyoxyethylene manufactured by Toho Chemical Industry Co. Ltd., Japan) was added 3-aminopropyl- dimethylsulfonium bromide hydrobromate in a proportion of 0.4 mg/ml to adjust the pH of the medium to 6.5.
Each 100 ml of the thus treated medium was separately charged into a Sakaguchi flask and was then sterilized. Subsequently, Streptomyces verticillus (ATCC No. 15003) was inoculated in the medium and was cultured at 27°C for 8 days with stirring at 130 r.p.m. Thereafter, the culture liquors (4.5 L) were collected and filtered to obtain 3.0 L of a filtrate (potency 38.8 mg/ml, total potency 416.4 mg). This culture filtrate was passed through and adsorbed on a column packed with 200 ml of Amberlite IRC-50 and was washed with water and was eluted with 0.5 N hydrochloric acid. 1.0 L of the eluate was neutralized, was passed through and adsorbed on a column packed with 100 ml of active carbon, was washed and was then eluted by use of a 1:1 (by volume) mixture of acetone - 0.02 N aqueous hydrochloric acid solution, and fractions active to Mycobacterium 607 were collected and concentrated to dryness. The resulting residue was dissolved in 5 ml of an 80 % aqueous methanol solution and was charged into a column packed with 30 ml of neutral alumina, followed by elution with an 80 % aqueous methanol solution. Subsequently, bleomycin-containing fractions were collected and concentrated to dryness to obtain 195 mg of bleomycin hydrochloride (potency 650.7 mcg/mg, total potency 172 mg). The yield from the culture filtrate was 30.5 %.
Therapeutic FunctionAntibiotic
Bleomycin hydrochloride Preparation Products And Raw materials
Raw materialsSOYBEAN MEAL-->D-Glucose monohydrate
Aluminum acetylacetonate METHYL ISOCYANOACETATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) N-BUTYLISOCYANIDE Tris(2,4-pentanedionato)chroMiuM(III) Topotecan hydrochloride Sibutramine hydrochloride Tramadol hydrochloride SALCOMINE Bleomycin Cupric acetylacetonate 1-AdaMantanethylaMine Ethyl isocyanoacetate TERT-BUTYL ISOCYANIDE Hydroxylamine hydrochloride Bleomycin sulfate Ferric acetylacetonate Bleomycin hydrochloride

Email:[email protected] [email protected]
Copyright © 2024 Mywellwork.com All rights reserved.