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| | DL-Dithiothreitol Basic information |
| Product Name: | DL-Dithiothreitol | | Synonyms: | DTT (RACEMIC);DL-THREO-2,3-DIHYDROXY-1,4-DITHIOLBUTANE;DL-THREO-1,4-DIMERCAPTO-2,3-BUTANEDIOL;DL-DTT;DITHIOTHREITOL, 1,4-;DITT;DL-2,3-DIHYDROXY-1,4-BUTANEDITHIOL;DL-1,4-DIMERCAPTO-2,3-BUTANEDIOL | | CAS: | 27565-41-9 | | MF: | C4H10O2S2 | | MW: | 154.25 | | EINECS: | 248-531-9 | | Product Categories: | Biochemistry;O-Substituted Sugars;Sugar Alcohols;Sugars;Reagents for Electrophoresis | | Mol File: | 27565-41-9.mol |  |
| | DL-Dithiothreitol Chemical Properties |
| Hazard Codes | Xn | | Risk Statements | 25-36/37/38-22 | | Safety Statements | 26-45-36 | | WGK Germany | 3 | | RTECS | EK1610000 | | F | 3-10-16-23 | | HS Code | 29309090 | | Toxicity | mouse,LD50,intramuscular,108mg/kg (108mg/kg),BEHAVIORAL: CONVULSIONS OR EFFECT ON SEIZURE THRESHOLD,Journal of Pharmacy and Pharmacology. Vol. 1, Pg. 576, 1949. |
| | DL-Dithiothreitol Usage And Synthesis |
| Chemical Properties | White solid | | Uses | DTT is a cell permeable protective agent for Thiol groups. | | Uses | Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins. | | Preparation | The preparation of DL-Dithiothreitol is as follows:To a round bottom flask was charged THPP (23mg, 0.11mmol, 1.10eq) and the basic buffer solution [tris-(hydroxymethyl)-aminomethane-CaCl2 based buffer; pH 8.00; 1.0mL]. The reaction mixture was stirred at room temperature for about 5 min and then to this homogenous solution was charged trans-4,5-Dihydroxy-1,2-dithiane (15.2mg, 0.10 mmol, 1.00eq.). The reaction mixture was stirred at room temperature until the reaction was complete (about 30 min). The product was readily identified by 1H-NMR analysis. | | Definition | ChEBI: D-1,4-dithiothreitol is a 1,4-dithiothreitol. It is an enantiomer of a L-1,4-dithiothreitol. | | Purification Methods | Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.] |
| | DL-Dithiothreitol Preparation Products And Raw materials |
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