Azure B

Azure B Basic information
Product Name:Azure B
Synonyms:N,N,N'-TRIMETHYLTHIONIN;3-(dimethylamino)-7-(methylamino)-phenothiazin-5-iumchloride;3-(dimethylamino)-7-(methylimino)-3h-phenothiazinehydrochloride;3-(dimethylamino)-7-(methylimino)-3h-phenothiazinhydrochloride;3-methylamino-7-dimethylaminophenzathoniumchloride;AZURE B, CERTIFIED (C.I. 52010);AZURE B, FOR MICROSCOPY;AZURE B CERTIFIED
CAS:531-55-5
MF:C15H16ClN3S
MW:305.82
EINECS:208-511-2
Product Categories:Phenothiazine
Mol File:531-55-5.mol
Azure B Structure
Azure B Chemical Properties
Melting point 205-210 °C (dec.)(lit.)
storage temp. -20°C Freezer
solubility H2O: soluble1mg/mL
Colour Index 52010
form Powder
color Dark green
Water Solubility Soluble
λmax648–655 nm
Merck 14,6058
BRN 3922630
Stability:Stable. Incompatible with strong oxidizing agents.
Biological ApplicationsAntimalarial; biofuel cell; medical devices; diagnosis of amyloid accumulation related diseases,diabetes,malignant melanoma; detecting oral cancer,cells,nucleic acids; treating avian influenza virus,nail infection,oral cavity infection
InChIKeyDNDJEIWCTMMZBX-UHFFFAOYSA-N
CAS DataBase Reference531-55-5(CAS DataBase Reference)
Safety Information
Safety Statements 22-24/25
WGK Germany 3
RTECS SO5550000
HS Code 32129000
MSDS Information
ProviderLanguage
SigmaAldrich English
ACROS English
ALFA English
Azure B Usage And Synthesis
Chemical Propertiesgreen crystalline powder
UsesUsed for staining reticulocytes. Azure B is used for preparing azure eosin stains for blood smears.
UsesBiological stain.
UsesAzure B is used for staining semi-thin sections of plant tissue. This dye is also useful in the NCCLS method for blood smears and Lillie's modified Nocht's method for paraffin sections.
DefinitionChEBI: An organic chloride salt having 3-(dimethylamino)-7-(methylamino)phenothiazin-5-ium as the counterion. It is used in making azure eosin stains for blood smear staining and is an active metabolite of methylene blue.
Biochem/physiol ActionsAzure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.
Purification MethodsAzure B has been twice recrystallised from H2O and dried at 100o/1hour in an oven. [Beilstein 27 III/IV 5151.]
Azure B Preparation Products And Raw materials
Raw materialsN,N-Dimethyl-1,4-phenylenediamine
Preparation ProductsAZURE A, CERTIFIED, PURE
Methylene Chloride BASIC BLUE 9 ZINC CHLORIDE DOUBLE SALT Azure B Methylene Blue Calcium chloride Methylene Blue trihydrate SALOR-INT L162914-1EA azur AZURE II-EOSIN,AZURE II EOSINATE Ferric chloride Choline chloride METHYLENE BLUE Ammonium chloride Polyvinyl chloride Potassium chloride DIMETHYLMETHYLENE BLUE HYDROCHLORIDE 2-​[[(2-​ethylphenyl)​(2-​hydroxyethyl)​amino]​methyl]​-​3,​3-​difluoro-Propanenitrile Cupric carbonate basic

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