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| | TRIS hydrochloride Basic information |
| Product Name: | TRIS hydrochloride | | Synonyms: | T-BAG WITH GRANULATE FOR 5L TSB;3-propanediol,2-amino-2-(hydroxymethyl)-hydrochloride;trizmahydrochloride(trishydrochloride);Tris(2-amino-2-hydroxymethyl)-1,3-propanediol, hydrochloride;Tris(hydroxymethyl)aminomethane hydrochloride, extra pure, 99+%;TrisHCl,mole-readyform,molecularbiologygrade;TRISHYDROXYMETHYLAMINOMETHANE HYDROCHLORIDE BIO-REFINED;TRIS(HYDROXYMETHYL)AMINOMETHANEHYDROCHLORIDE,BIOTECHGRADE | | CAS: | 1185-53-1 | | MF: | C4H12ClNO3 | | MW: | 157.6 | | EINECS: | 214-684-5 | | Product Categories: | Buffer;1185-53-1 | | Mol File: | 1185-53-1.mol |  |
| | TRIS hydrochloride Chemical Properties |
| Melting point | 150-152 °C | | Boiling point | 225℃[at 101 325 Pa] | | density | 1.05 g/mL at 20 °C | | vapor pressure | 0Pa at 20℃ | | storage temp. | room temp | | solubility | H2O: 4 M at 20 °C, clear, colorless | | form | crystalline | | color | clear colorless (40 % (w/w) in H2O) solution | | PH Range | 7.0 - 9.0 | | PH | 2.5-4.0 (25℃, 4M in H2O) | | pka | 8.1(at 25℃) | | Water Solubility | SOLUBLE | | Sensitive | Hygroscopic | | λmax | λ: 260 nm Amax: 0.12
λ: 280 nm Amax: 0.08 | | BRN | 3675235 | | Stability: | Stable. Incompatible with strong oxidizing agents, bases, moisture. | | InChIKey | QKNYBSVHEMOAJP-UHFFFAOYSA-N | | LogP | -3.6 at 20℃ | | CAS DataBase Reference | 1185-53-1(CAS DataBase Reference) | | EPA Substance Registry System | 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1) |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26-36 | | WGK Germany | 3 | | RTECS | TY2900000 | | F | 3 | | TSCA | Yes | | HS Code | 29221980 |
| | TRIS hydrochloride Usage And Synthesis |
| Chemical Properties | TRIS hydrochloride is a white crystalline powder that has a pKa of 8.08 at 25°C and pH range of 7.0 to 9.0 that coincides with the physiological pH of most living organisms because of which it is commonly used as a component of buffer solutions in biology, biochemistry and molecular biology applications. Tris salts are also used for crystallization of proteins at various pH values. Neither Tris base nor Tris hydrochloride by itself provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering. | | Uses | TRIS hydrochloride is a high quality buffer component used in SDS-PAGE. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. It is also used in the robust generation of transgenic mice. | | Application | TRIS hydrochloride is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer. | | Preparation | The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid. | | General Description | TRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution. | | Biological Activity | Commonly used laboratory reagent | | Purification Methods | Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.] |
| | TRIS hydrochloride Preparation Products And Raw materials |
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