EC 3.1.4.4;EC 3.1.4.4 TYPE II;EC 3.1.4.4 TYPE III;EC 3.1.4.4 TYPE IV;EC 3.1.4.4 TYPE VI;EC 3.1.4.4 TYPE VII;LECITHINASE D TYPE II;LECITHINASE D TYPE III
Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).
Uses
Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies.
General Description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.
Biochem/physiol Actions
Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
EC 3.1.4.4 TYPE IV Preparation Products And Raw materials
