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| Product Name: | PULLULANASE | | Synonyms: | pullulanase from bacillus sp.;pullulanase from klebsiella pneumoniae;PULLULANASE FROM BACILLUS SP., POWDER, ~ 1 U/MG;PULLULANASE FROM BACILLUS*ACIDOPULLULYTI;pullulanase from bacillus acidopullulyticus;PULLULANASE SUSPENSION, FROM KLEBSIELLA PNEUMONIAE;Amylopectin 6-gluconohydrolase, Limit dextrinase;PromozymeTM 400L, Pullulan 6-glucano-hydrolase | | CAS: | 9075-68-7 | | MF: | NULL | | MW: | 0 | | EINECS: | 232-983-9 | | Product Categories: | | | Mol File: | Mol File | ![PULLULANASE Structure]() |
| | PULLULANASE Chemical Properties |
| density | 1.25 g/mL at 25 °C(lit.) | | vapor pressure | 0Pa at 25℃ | | storage temp. | 2-8°C | | form | lyophilized powder | | Water Solubility | 100g/L at 25℃ | | LogP | -1.3 at 20℃ | | CAS DataBase Reference | 9075-68-7 | | EPA Substance Registry System | Pullulanase (9075-68-7) |
| | PULLULANASE Usage And Synthesis |
| Description | As an an amylolytic exoenzyme, Pullulanase is one of the most essential endo-type enzyme that degrades pullulan. It is found in plants and microorganisms, in which it is synthesized as an extracellular, cell surface-anchored lipoprotein by the Gram-negative bacterium such as Bacillus brevis, genus Klebsiella. Type I pullulanases specifically cleaves α-1,6-glucosidic linkages in polysaccharides, such as starch, amylopectin, glycogen, dextrin, pullulan and related oligosaccharides, whilst type II pullulanases are effective to attack α-1,6-glucosidic linkages. This enzyme is applied on a large scale in glucose and maltose syrup industries, where it is combined with the activities of other enzymes, such as β-amylase and isoamylase to manufacture maltose and oligosaccharides. Besides, it commercially serves as an industrial enzyme preparation in various reactions, such as the production of branched cyclodextrins, where pullulanase can catalyze the condensation reaction. Pullulanase is also applied as a processing aid in grain processing biotechnology for the production of ethanol and sweeteners.
| | References | https://en.wikipedia.org/wiki/Pullulanase
http://www.sigmaaldrich.com/catalog/product/sigma/e2412?lang=en®ion=US
https://www.hindawi.com/journals/er/2012/921362/
http://www.amano-enzyme.co.jp/eng/enzyme/15.html
http://www.ssjournals.com/index.php/ijbr/article/view/610
| | Uses | One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25°C. | | Uses | Pullulanase is a glucanase that degrades pullulan. It is produced by the Gram-negative bacterium Klebsiella and is used for starch-debranching as well as to study polysaccharide utilization. | | Biochem/physiol Actions | Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. |
| | PULLULANASE Preparation Products And Raw materials |
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