| | OPD EASY-tablets Basic information |
| | OPD EASY-tablets Chemical Properties |
| Melting point | 258 °C (dec.)(lit.) | | storage temp. | 2-8°C | | solubility | H2O: 1 tablet/10 mL, clear, colorless | | form | tablet | | color | white to off-white | | PH | 1.5 | | Water Solubility | Slightly soluble in water; freely soluble in alcohol, chloroform or ether | | BRN | 3912045 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | IARC | 2B (Vol. 123) 2020 | | EPA Substance Registry System | 1,2-Phenylenediamine dihydrochloride (615-28-1) |
| | OPD EASY-tablets Usage And Synthesis |
| Chemical Properties | white to light pink powder | | Uses | o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm. | | Uses | o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm. | | Uses | o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA). | | General Description | Brownish-yellow crystals. Slightly soluble in water. Used in the manufacture of dye. | | Air & Water Reactions | Slightly soluble in water. | | Reactivity Profile | Acidic organic/inorganic salts, such as OPD EASY-tablets, are generally soluble in water. The resulting solutions contain moderate to high concentrations of hydrogen ions and have pH's of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid. They usually do not react as either oxidizing agents or reducing agents but such behavior is not impossible. Many of these compounds catalyze organic reactions. | | Purification Methods | Crystallise the salt from dilute HCl (60mL conc HCl, 40mL water, with 2g stannous chloride), after treatment of the hot solution with charcoal by adding an equal volume of conc HCl and cooling in an ice-salt mixture. The crystals are washed with a small amount of conc HCl and dried in a vacuum desiccator over NaOH. [Beilstein 13 IV 38.] |
| | OPD EASY-tablets Preparation Products And Raw materials |
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