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| | Hygromycin B Basic information |
| Product Name: | Hygromycin B | | Synonyms: | antihelmycin;hydromycinb;hygromix2.4;hygromix-8;hygrovetine;Hygromycin Solution;Hygromix;5-O-(2,3-O-(6-Amino-6-desoxyheptapyranosyliden)-beta-D-talopyranosyl)-desoxy-N'-methyl-D-streptamin | | CAS: | 31282-04-9 | | MF: | C20H37N3O13 | | MW: | 527.52 | | EINECS: | 250-545-5 | | Product Categories: | antibiotic;Inhibitors | | Mol File: | 31282-04-9.mol |  |
| | Hygromycin B Chemical Properties |
| Melting point | 160-180°C | | alpha | D20 +20.2° (c = 1 in water) | | Boiling point | 608.13°C (rough estimate) | | density | 1.4252 (rough estimate) | | refractive index | 1.6300 (estimate) | | storage temp. | 2-8°C | | solubility | H2O: 50 mg/mL As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days. | | pka | 7.1, 8.8(at 25℃) | | form | powder | | color | faintly brown to brown | | PH | pH (25g/l, 25℃ ) : 9.5～12.5 | | Water Solubility | Miscible with water, methanol, buffer solution and ethanol. Immiscible with less polar solvents. | | Merck | 14,4852 | | BRN | 6755837 | | Stability: | Hygroscopic | | EPA Substance Registry System | Hygromycin B (31282-04-9) |
| | Hygromycin B Usage And Synthesis |
| Description | Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus. It is active against E. coli (MIC = 150 μg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis. Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph. | | Chemical Properties | White to off-white lyophilized powder | | Uses | antibacterial, anthelmintic; LD50 ip (rat) 63mg/Kg | | Uses | Hygromycin B, a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome | | Uses | Hygromycin B is an aminoglycoside and a selective antibiotic agent for the hph gene. Hygromycin B inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S rib
osome | | Uses | Hygromycin B is a trisaccharide aminoglycoside with a terminal cyclitol produced by Streptomyces hygroscopicus, reported by researchers at Eli Lily in the early 1950s. Hygromycin B belongs to the destomycin family of aminoglycosides which contain a unique cyclic structure linking the terminal saccharide. Hygromycin B exhibits broad spectrum antibiotic and anthelmintic activity, and is used as a feed additive for swine and poultry. Hygromycin B inhibits polypeptide synthesis by binding peptidyl-tRNA and preventing translocation by Elongation Factor 2. | | General Description | Amorphous solid or tan powder. | | Air & Water Reactions | Water soluble. | | Reactivity Profile | Weakly basic. | | Fire Hazard | Flash point data for Hygromycin B are not available; however Hygromycin B is probably combustible. | | Biochem/physiol Actions | Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells. | | Safety Profile | Poison by
intraperitoneal route. Mutation data
reported. When heated to decomposition it
emits toxic fumes of Nox | | storage | +4°C | | references | [1] brodersen de, clemons wm jr, carter ap, et al. the structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin b on the 30s ribosomal subunit. cell, 2000, 103 (7): 1143-1154.
[2] ganoza mc, kiel mc. a ribosomal atpase is a target for hygromycin b inhibition on escherichia coli ribosomes. antimicrob agents chemother, 2001, 45 (10): 2813-2819. |
| | Hygromycin B Preparation Products And Raw materials |
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