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| | Fluorescamine Basic information |
| | Fluorescamine Chemical Properties |
| Melting point | 153-157 °C (lit.) | | Boiling point | 381.08°C (rough estimate) | | density | 1.2661 (rough estimate) | | refractive index | 1.5447 (estimate) | | Fp | -17 °C | | storage temp. | room temp | | solubility | acetonitrile: soluble | | form | powder | | color | off-white to yellow | | Water Solubility | Soluble in acetone, ethanol, chloroform, dimethylsulfoxide and acetonitrile. Slightly soluble in water. | | Merck | 14,4158 | | BRN | 921143 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | CAS DataBase Reference | 38183-12-9(CAS DataBase Reference) | | NIST Chemistry Reference | Spiro[furan-2(3h),1'(3'h)-isobenzofuran]-3,3'-dione, 4-phenyl-(38183-12-9) |
| | Fluorescamine Usage And Synthesis |
| Chemical Properties | white powder | | Uses | Analytical reagent. | | Uses | For fluorescent detection of amino acids, peptides and proteins.Fluorescamine plays an important role as a reagent for the detection of amines, peptides and proteins. It is also used as a substrate for measuring protease activity. It is utilized in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by HPLC. It finds application in the determination of lisinopril in human plasma and urine. | | Uses | Fluorescamine is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis.
| | Synthesis Reference(s) | Journal of the American Chemical Society, 94, p. 5927, 1972 DOI: 10.1021/ja00771a084 | | Purification Methods | Fluram is a non-fluorescent reagent that reacts with primary amines to form highly fluorescent compounds. Purify it by dissolving (~1g) in Et2O/*C6H6 (1:1, 180 mL), washing with 1% aqueous NaHCO3 (50mL), drying (Na2SO4), and evaporating in a vacuum. Dissolve the residue in warm CH2Cl2 (5mL), dilute with Et2O (12mL) and refrigerate. Collect the solid and dry it in a vacuum. IR (CHCl3): 1810, 1745, 1722, 1625 and 1600 cm-1, and 1HNMR (CDCl3): 8.71 (s, -OHC=). [Weigele et max al. J Am Chem Soc 94 5927 1972, Weigele et al. J Org Chem 41 388 1976, Lai Methods Enzymol 47 236 1977.] Forskolin (Colforsin, Coleonol, 5-[acetyloxy]-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7, 10a-penta-methyl-[3R -{3 |
| | Fluorescamine Preparation Products And Raw materials |
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