| Chemical Properties | white to light beige fine crystalline powder |
| Uses | N-tert-Butyl-α-phenylnitrone has been used:
- as a component of Dneasy Blood&Tissue buffer to preserve the oxidized state of DNA extracted from human non-tumorigenic epithelial breast (MCF10A) cells
- as a free radical scavenger of reactive oxygen species (ROS) in microglial (MG) cell lines
- to attenuate fibroblast senescence in unstable oral squamous cell carcinomas (GU-OSCC)
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| Uses | Spin trap reagent for carbon- or oxygen- or nitrogen-centered free radicals. |
| Uses | N-tert-Butyl-a-phenylnitrone is a commonly-used free radical trap. It contains radical scavenging activity and an ability to inhibit Cox-2 (cyclooxygenase-2). N-tert-Butyl-a-phenylnitrone is an antioxidant that has been shown to act as a protective agent in several experimental models of neurodegenerative disorders. N-tert-Butyl-a-phenylnitrone inhibits lipid peroxidation in rat liver microsomes. It also prevents the induction of inducible nitric oxide synthase (iNOS) by reactive oxygen species. |
| Synthesis Reference(s) | Tetrahedron, 48, p. 8677, 1992 DOI: 10.1016/S0040-4020(01)89443-6 |
| General Description | Most commonly-used free radical trap. An antioxidant that has been shown to act as a protective agent in several experimental models of neurodegenerative disorders. Inhibits lipid peroxidation in rat liver microsomes. Also prevents the induction of inducible nitric oxide synthase (iNOS) by reactive oxygen species. |
| Biochem/physiol Actions | Product does not compete with ATP. |
| Purification Methods | Crystallise PBN from hexane. It is a free radical trap. [cf Janzen Methods Enzymology 105 188 1984, Beilstein 7 IV 519.] |
