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| | Direct Blue 14 Basic information |
| | Direct Blue 14 Chemical Properties |
| Melting point | >300 °C (lit.) | | density | 1.007 g/mL at 20 °C | | storage temp. | dry at room temperature | | solubility | H2O: soluble10mg/mL | | Colour Index | 23850 | | form | Powder | | color | Dark greenish-brown | | PH | 9.8 (10g/l, H2O, 20℃) | | Water Solubility | 10 g/L (25 ºC) | | Merck | 14,9792 | | BRN | 4360496 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | CAS DataBase Reference | 72-57-1(CAS DataBase Reference) | | IARC | 2B (Vol. 8, Sup 7) 1987 | | EPA Substance Registry System | Trypan blue (72-57-1) |
| | Direct Blue 14 Usage And Synthesis |
| Chemical Properties | Trypan Blue is a dark Blue crystalline solid or
powder. | | Chemical Properties | blueish grey powder | | Uses | Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells. | | Uses | Biological stain. | | Uses | Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites. | | Preparation | 3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled. | | Definition | ChEBI: An organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid). | | General Description | Bluish-gray to dark blue powder. | | Air & Water Reactions | Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water. | | Reactivity Profile | Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids. | | Health Hazard | ACUTE/CHRONIC HAZARDS: Direct Blue 14 may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition Direct Blue 14 emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide. | | Fire Hazard | Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible. | | Biochem/physiol Actions | Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells. | | Safety Profile | Suspected carcinogen
with experimental carcinogenic,
neoplastigenic, and tumorigenic data. Poison
by intraperitoneal, intravenous, and
subcutaneous routes. Experimental
teratogenic and reproductive effects.
Mutation data reported. When heated to
decomposition it emits very toxic fumes of
NOx NazO, and SOx | | Potential Exposure | Used in dyeing textiles; leather and
paper; as a biological stain. | | Shipping | UN2811 Toxic solids, organic, n.o.s., Hazard
Class: 6.1; Labels: 6.1-Poisonous materials, Technical
Name Required. UN3143 dyes, solid, toxic, n.o.s. or dye
intermediates, solid, toxic, n.o.s., Hazard Class: 6.1;
Labels: 6.1-Poisonous materials, Technical Name Required. | | Properties and Applications | blue. Blue gray powder. Soluble in water for blue, slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid in dark green light for blue, diluted into a red light blue; In the light of nitric acid for palm gray solution. The dye solution to join a red sulfuric acid 10% change; Add thick sodium hydroxide solution for red light purple.
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Standard
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Acid Resistance
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Alkali Resistance
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Light Fastness
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Soaping
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Water
|
|
Fading
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Stain
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Fading
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Stain
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|
ISO
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4-5
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4
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1-2
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2
|
|
2
|
|
|
AATCC
|
5
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2
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1-2
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2
|
|
1
|
|
| | Incompatibilities | Dust may form explosive mixture with
air. Trypan Blue is incompatible with strong oxidizing
agents. Incompatible with oxidizers (chlorates, nitrates,
peroxides, permanganates, perchlorates, chlorine, bromine,
fluorine, etc.); contact may cause fires or explosions. Keep
away from alkaline materials, strong bases, strong acids,
oxoacids, epoxides. Azo compounds can detonate. This
applies in particular to organic azides that have been sensitized
by the addition of metal salts or strong acids. Toxic
gases are formed by mixing materials of this class with
acids, aldehydes, amides, carbamates, cyanides, inorganic
fluorides, halogenated organics, isocyanates, ketones,
metals, nitrides, peroxides, phenols, epoxides, acylhalides,
and strong oxidizing or reducing agents. Flammable gases
are formed by mixing materials in this group with alkali
metals. Explosive combinations can occur with strong oxidizing
agents, metal salts, peroxides, and sulfides. Trypan
Blue is sensitive to prolonged exposure to heat. | | Waste Disposal | Consult with environmental
regulatory agencies for guidance on acceptable disposal
practices. Generators of waste containing this contaminant
(≥100 kg/mo) must conform with EPA regulations governing
storage, transportation, treatment, and waste
disposal. |
| | Direct Blue 14 Preparation Products And Raw materials |
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