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| Product Name: | PR-619 | | Synonyms: | PR-619;2,6-DiaMino-3,5-dithiocyanopyridine;3,5-dithiocyanatopyridine-2,6-diaMine;Thiocyanic acid, C,C'-(2,6-diamino-3,5-pyridinediyl) ester;2,6-Diamino-3,5-dithiocyanopyridine PR 619;PR 619 2,6-Diamino-3,5-dithiocyanopyridine;2,6-Diaminopyridine-3,5-bis(thiocyanate);PR-619;PR619 | | CAS: | 2645-32-1 | | MF: | C7H5N5S2 | | MW: | 223.28 | | EINECS: | 200-256-5 | | Product Categories: | Inhibitors | | Mol File: | 2645-32-1.mol |  |
| | PR-619 Chemical Properties |
| Melting point | 210℃ | | Boiling point | 406.0±45.0 °C(Predicted) | | density | 1.61±0.1 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | DMSO: soluble5mg/mL (clear solution) | | pka | -0.19±0.50(Predicted) | | form | powder | | color | white to beige | | Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 month. |
| | PR-619 Usage And Synthesis |
| Description | PR-619 (2645-32-1) is a nonselective, reversible inhibitor of cysteine-reactive deubiquitinating enzymes (DUBs; 5-20 μM)1. PR-619 is highly useful for preserving ubiquitinated proteins during cell lysis. DUBs become unregulated in cell lysates and will act quickly to deubiquitinate proteins of interest during cell lysis. To preserve maximum amounts of ubiquitinated protein, include PR-619 (50-100 μM) in lysis buffers. PR-619 is also useful for interrogating the role of DUBs in intact cell systems2, and treatment of cells results in an overall accumulation of ubiquitinated proteins and many other phenotypes. | | Uses | PR 619 is a broad-range DUB inhibitor. A selective and general inhibitior of cellular DUB activity without direct impairment of proteasomal proteolysis. Used in the preparation of thiazolopyridines an
d bisthiazolopyridines. | | Uses | PR-619 has been used:
- as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells
- as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction
- in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays
| | Biochem/physiol Actions | PR-619 is a cell permeable broad spectrum deubiquitylating enzymes (DBUs) inhibitor. PR-619 induces the accumulation of polyubiquitylated proteins in cells without directly affecting proteasome activity. | | storage | Store at -20°C | | References | 1) Altun?et al. (2011),?Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes; Chem. Biol.,?18?1401
2) Seiberlich?et al. (2012),?The small molecule inhibitor PR-619 of deubiquitinating enzymes affects the microtubule network and causes protein aggregate formation in neural cells: implications for neurodegenerative diseases; Biochim. Biophys. Acta,?1823?2057 |
| | PR-619 Preparation Products And Raw materials |
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