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| Product Name: | Chapso | | Synonyms: | CHAPSO;3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANESULFONATE;3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANE SULPHONATE;1-Propanaminium, 2-hydroxy-N,N-dimethyl-3-sulfo-N-3-(3.alpha.,5.beta.,7.alpha.,12.alpha.)-3,7,12-trihydroxy-24-oxocholan-24-ylaminopropyl-, inner salt;CHAPSO,ELECTROPHORESISGRADE;3-((3-cholamidopropyl)dimethylammonio)-2-hydroxy-;CHAPSO 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate;CHAPSO SIGMAULTRA | | CAS: | 82473-24-3 | | MF: | C32H58N2O8S | | MW: | 630.88 | | EINECS: | 617-338-5 | | Product Categories: | detergent;Glycon Biochem | | Mol File: | 82473-24-3.mol |  |
| | Chapso Chemical Properties |
| Melting point | 184-186 °C(lit.) | | density | 1.0453 (rough estimate) | | refractive index | 1.6900 (estimate) | | storage temp. | -20°C | | solubility | H2O: 10 mg/mL at 20 °C, clear, colorless | | form | White solid | | color | White crystalline | | Odor | Odorless | | Water Solubility | 10 g/l at 20 °C | | Merck | 13,2052 | | BRN | 5842642 | | CAS DataBase Reference | 82473-24-3(CAS DataBase Reference) |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26-36 | | WGK Germany | 3 | | F | 3-10 | | HS Code | 2924297099 |
| | Chapso Usage And Synthesis |
| Chemical Properties | Chapso is a white crystalline powder. It is a zwitterionic detergent similar to Chaps, however, Chapso is more soluble and particularly better for the solubilization of integral membrane proteins. | | Uses | Chapso is a nondenaturing biological detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins. | | Application | 3-(cholamidopropyl)(dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO) may be used in bicelle preparation for β2-adrenergic receptor reconstitution.
CHAPSO has been used:
in a study to assess the processes of liposome solubilization and reconstitution of two transport proteins
in a study to investigate a novel bicelle system for orienting macromolecules over a wide temperature range
as a mixture with dilauroyl phosphatidylcholine (DLPC) in solid-state nuclear magnetic resonance (NMR) technique to study membrane-associated proteins
A nondenaturing zwitterionic detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins. | | General Description | CHAPSO is a non-denaturing zwitterionic bile salt derivative, derived from Chaps by the addition of a functional hydroxyl group. | | Purification Methods | Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] |
| | Chapso Preparation Products And Raw materials |
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