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| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Basic information |
| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Chemical Properties |
Melting point | 0.05°C | Boiling point | 586.77°C (rough estimate) | density | 1.06 g/mL at 20 °C | vapor pressure | <0.01 hPa (20 °C) | refractive index | n20/D 1.492 | Fp | >230 °F | storage temp. | Store at +5°C to +30°C. | solubility | Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly), Water (Slightly) | form | Liquid | color | Yellow | PH | 5.0-8.0 (10g/l, H2O, 20℃) | Water Solubility | Miscible with water. | Merck | 13,6793 | BRN | 2315025 | CAS DataBase Reference | 9036-19-5(CAS DataBase Reference) | EPA Substance Registry System | Polyethylene glycol mono(1,1,3,3-tetramethylbutyl)phenyl ether (9036-19-5) |
| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Usage And Synthesis |
Uses | Triton? X-100 has been used:
- In immunohistochemistry for staining the Flat-mount retinas
- Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
- To permeabilise cells during Immunofluorescent microscopic studies
- As a positive control in LDH assay to determine the cell membrane integrity
- For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
- For the preparation of outer membrane protein exctract
- As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
- In the treatment of tissue sections for Immunofluorescence labeling
| Uses | 0·3% Triton? X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis. | Uses | Triton?-X has been used:
- In the permeabilization of cells for immunofluorescence staining
- As a component of lysis buffer in western blot analysis
- As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope
| General Description | Octophenolpoly(ethyleneglycolether)n Especially used for purification in membrane research Triton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification. | Biochem/physiol Actions | Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. | Safety Profile | Moderately toxic by ingestion.When heated to decomposition it emits acrid smoke andirritating fumes. | Safety Profile | Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits acridsmoke and irritating fumes. |
| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Preparation Products And Raw materials |
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