| Uses | Dansyl chloride or 5-Dimethylaminonaphthalene-1-sulfonyl chloride (CAS 605-65-2) is a fluorogenic reagent used for protein, N-terminal amino acid and peptide detection by reverse phase high performance liquid chromatography (HPLC). It is also used to determine the degree of labeling in chymotrypsin and ovalbumin. It acts as a precursor for the synthesis of other derivatives like dansyl amide.
Dansyl chloride is non-fluorescent until it reacts with amines. The resulting Dansyl amides have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl chloride an important tool for biophysical studies.
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| Chemical Properties | yellow to orange-yellow crystalline powder |
| Uses | Dansyl chloride is a fluorogenic reagent used for protein, N-terminal amino acid and peptide detection by reverse phase high performance liquid chromatography (HPLC). It is also used to determine the degree of labeling in chymotrypsin and ovalbumin. It acts as a precursor for the synthesis of other derivatives like dansyl amide. |
| Uses | In fluorescent labelling, column chromatography, HPLC. |
| Uses | A fluorogenic reagent for N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC. |
| Uses | Reagent for fluorescent labelling of amines, amino acids and proteins. |
| Definition | ChEBI: Dansyl chloride is an aminonaphthalene and a sulfonic acid derivative. |
| General Description | Dansyl chloride is a fluorogenic reagent which is typically employed for the derivatization of amino acids. |
| Biochem/physiol Actions | Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins. Dansyl chloride is used for derivatization of free amino acids and amines in neural samples and biological fluids. It is used to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization. |
| Safety Profile | Poison by intravenous
route, When heated to decomposition it
emits very toxic fumes of Cland NOx |
