| Description | Phytases are phosphatases able to hydrolyze O–P bonds in phytic acid liberating
inorganic phosphate. Phytases can be grouped according to the attack on the
hexaphosphoric ester into 3-phytase (myo-inositol-hexakisphosphate 3-phosphohydrolase,
EC 3.1.3.8) as well as 4-phytase (myo-inositol-hexakisphosphate 4-
phosphohydrolase, EC 3.1.3.26), releasing the phosphate at the corresponding
position at the inositol ring.
Phytic acid is used by plants to store different type of anions (Cu2+, Fe2+/3+,
Ca2+, Mg2+, etc.). The resulting salts are known as phytates. Nonruminant animals
do not have the enzymatic ability to hydrolyze phytates; therefore, phosphate and
minerals are not absorbed, but rather they pass through the intestinal tract undigested.
The addition of phytases to food and feed does therefore enhance the
availability of phosphate and minerals bound in phytates.
More than 20 years ago, the first commercial phytase product for feed, Natuphos,
was released. Nowadays, several other phytase products are available on
the market for the improvement of animal feed, such as Ronozyme from Novozymes
and Finase from AB Enzymes. The range of applications in nonruminant feed is large, as discussed in several reviews on the usage of phytases in the fodder
of pigs, poultry, and fish. Additionally, phytase might also be
used in food for the improvement of the nutritional value of cereal food products
by degrading phytate.
Commercial recombinant production of phytases occurs generally, if not
exclusively, in ascomycetes, whereas the phytase genes originate from
different phyla: bacteria, ascomycetes, and basidiomycetes (Peniophora lycii).
Industrial production takes place in liquid media in stirred bioreactors on a
6 9 120 m3 scale. In addition, the cultivation of phytase producing filamentous
fungi in solid-state or solid-substrate fermentation systems has also been
studied, but it lacks industrial adaptability. Another opportunity for the production
of phytases is the usage of transgenic plants, such as maize, rice, soybean, and
wheat. |
