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Disodium 4-nitrophenylphosphate

Disodium 4-nitrophenylphosphate Basic information
Product Name:Disodium 4-nitrophenylphosphate
Synonyms:Disodium 5-nitrophenylphosphate;Disodium 4-nitrophenylphosphate ,99%;4-Nitrophenyl phosphate disodium salt hexahydrate p.a.;p-NPP (p-Nitrophenyl phosphate, disodium, hexahydrate);PNPP, 2NA;PNP-PHOS DINA;PNPP LIQUID SUBSTRATE SYSTEM;P-NITROPHENYL PHOSPHATE DISODIUM
CAS:4264-83-9
MF:C6H7NNaO6P
MW:243.09
EINECS:224-246-5
Product Categories:Enzyme substrates;Substrates;Biological Buffers;Buffer Convenience Packaging;Buffer SolutionsBiological Buffers;Buffers A to Z
Mol File:4264-83-9.mol
Disodium 4-nitrophenylphosphate Structure
Disodium 4-nitrophenylphosphate Chemical Properties
Melting point >300 °C(lit.)
density 1.751 at 20℃
vapor pressure 15Pa at 20-50℃
storage temp. -20°C
solubility H2O: 1 tablet/3 mL 25 mM, clear, light yellow
form tablets
color white to pale yellow
Water Solubility Miscible with water. Immiscible with ethanol and acetone.
BRN 5324661
Stability:Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.
InChIKeyKAKKHKRHCKCAGH-UHFFFAOYSA-L
LogP-2.16 at 22℃ and pH6.7
Surface tension69.34mN/m at 1.051g/L and 20℃
CAS DataBase Reference4264-83-9(CAS DataBase Reference)
EPA Substance Registry SystemPhosphoric acid, mono(4-nitrophenyl) ester, disodium salt (4264-83-9)
Safety Information
Hazard Codes Xi,Xn
Risk Statements 36/38-36/37/38-48/22-41-38-21/22
Safety Statements 26-36-24/25-23-36/37/39
WGK Germany 3
F 10-21
TSCA Yes
HS Code 29199000
MSDS Information
ProviderLanguage
Disodium 4-nitrophenylphosphate English
SigmaAldrich English
ALFA English
Disodium 4-nitrophenylphosphate Usage And Synthesis
Chemical PropertiesWhite crystalline
UsesPNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures.
General DescriptionExcellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.
Biochem/physiol ActionsPrimary TargetSubstrate for phosphatase
Purification MethodsDissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.]
Disodium 4-nitrophenylphosphate Preparation Products And Raw materials
Raw materialsPhosphorus oxychloride-->4-Nitrophenol-->PARAOXON-->P-NITROPHENYL PHOSPHATE DI(TRIS) SALT-->BIS(4-NITROPHENYL)PHOSPHORIC ACID SODIUM SALT
Clodronate disodium Tylosin phosphate AMPHISOL A Diphenylphosphoryl azide ROCK PHOSPHATE Starch, hydrogen phosphate, 2-hydroxypropyl ether Disodium 4-nitrophenylphosphate 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE TRI-AMMONIUM PHOSPHATE TRIHYDRATE 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt PHENYLPHOSPHONIC ACID DISODIUM SALT PHOSPHATE STANDARD Diphenyl chlorophosphate phosphate Nitrophenyl PHENYL PHOSPHATE DISODIUM SALT Pamidronate disodium salt Disodium phenyl phosphate dihydrate
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