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| | Rhodamine 123 Basic information |
| Product Name: | Rhodamine 123 | | Synonyms: | Rhodamine methyl ester hydrochloride;Rhodamin 123 Chloride, laser grade;2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester monohydrochloride;Benzoic acid, 2-(6-amino-3-imino-3H-xanthen-9-yl)-, methyl ester, monohydrochloride;Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride;Rhodamine 123 ,98%;RHODAMINE 123, 99+%, PURE;Rhodamine 123, pure | | CAS: | 62669-70-9 | | MF: | C21H17ClN2O3 | | MW: | 380.83 | | EINECS: | 263-687-8 | | Product Categories: | Xanthene;Amines;Aromatics;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Fluorescent Labels & Indicators;ROX | | Mol File: | 62669-70-9.mol |  |
| | Rhodamine 123 Chemical Properties |
| Melting point | 235 °C | | density | 1.2224 (rough estimate) | | refractive index | 1.6500 (estimate) | | storage temp. | Hygroscopic, -20°C Freezer, Under inert atmosphere | | solubility | ethanol: 20 mg/mL, clear, red | | form | Powder | | color | Red-brown to brown or dark green | | Water Solubility | Partially soluble | | λmax | 501 nm, 507 nm | | BRN | 6030951 | | Stability: | Hygroscopic | | Major Application | Colored bubbles; color
filters; liquid crystal displays; light-emitting diodes;
luminescent materials;nanophotonic composites;
paints; semiconductor electrodes;solar cells | | Biological Applications | Measuring membrane potential; detecting cancer cells,spores,prostate cancer,stress biomarkers; treating disc degenerative disease,epilepsy,erectile dysfunction; apoptosis assays; tumor cell multidrug resistance assay; implantable | | InChIKey | MYFATKRONKHHQL-UHFFFAOYSA-N | | CAS DataBase Reference | 62669-70-9(CAS DataBase Reference) | | EPA Substance Registry System | Xanthylium, 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]-, chloride (62669-70-9) |
| Hazard Codes | Xn | | Risk Statements | 22 | | Safety Statements | 22-24/25 | | WGK Germany | 3 | | RTECS | ZE0883000 | | HS Code | 32049010 |
| | Rhodamine 123 Usage And Synthesis |
| Description | Rhodamine 123 is a membrane-permeable cationic dye that is readily accumulated within living cells. It is a substrate for the efflux pump P-glycoprotein (P-gp; also known as multidrug resistance protein 1 and ABCB1) and is rapidly exported from cells with functional P-gp. As P-gp is expressed on a population of stem cells known as the side population, rhodamine 123 is used to detect this group of stem cells. Rhodamine 123 also accumulates within mitochondria due to its positive charge and can inhibit oxidative phosphorylation. Rhodamine 123 has excitation/emission maxima of 507/529 nm. | | Chemical Properties | red-brown to brown or dark green powder | | Uses | Rhodamine derivative, a fluoresent dye-labeled compound. | | Uses | Fluorescent dye most commonly used as functional reporter in flow cytometry. | | Uses | Rhodamine-123
lycoprotein (P-gp) functional efflux activity in vivo. Rhodamine-123
PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). | | Uses | Useful as a laser dye and to have selective cell growth effects. Mitochondrial specific fluorescent dye and substrate for P-glycoprotein. | | Uses | Rhodamine-123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Rhodamine 123 (R123), as a typical of P-glycoprotein substrate, was widely used to quantify P-glycoprotein (P-gp) functional efflux activity in vivo. Rhodamine-123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). | | in vitro | rhodamine 123, which was identified as a member of the rhodamine family of flurone dyes, was used to examine membrane transport by the abcb1 gene product, mdr1. previous study determined the λmax for excitation and emission for rhodamine 123 in commonly used solvents and extraction buffers, indicating that the fluorescence of rhodamine 123 was highly dependent on the chemical environment. the optimal parameters are 1% methanol in hbss. in addition, the uptake of rhodamine 123 into cells was via both passive and active processes, and this occurred mainly by oatp1a2-mediated transport. furthermore, this previous study quantified the intracellular sequestration and metabolism of rhodamine 123, showing that these were both cell line-dependent factors that might influence the interpretation of transport assays [1]. | | references | [1] samantha forster et al. characterization of rhodamine-123 as a tracer dye for use in in vitro drug transport assays. plos one. 2012; 7(3): e33253. |
| | Rhodamine 123 Preparation Products And Raw materials |
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