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| | PYRONIN Y Basic information |
| Product Name: | PYRONIN Y | | Synonyms: | Pyronin Y,Pyronin G, PyroninJ, PyroninY;Pyronin G, C.I. 45005, for microscopy;Pyronin G (Pyronin Y);Pyronin y(G);Pyronin G, PyroninJ;Pyronin G, PyroninJ, PyroninY;Pyronin Y (CI x 45005);Pyronin Y,pure,high purity biological stain | | CAS: | 92-32-0 | | MF: | C17H19ClN2O | | MW: | 302.8 | | EINECS: | 202-147-8 | | Product Categories: | Xanthene | | Mol File: | 92-32-0.mol |  |
| | PYRONIN Y Chemical Properties |
| Melting point | 250-260 °C(lit.) | | storage temp. | room temp | | solubility | Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve | | Colour Index | 45005 | | form | Powder/Solid | | color | Pink | | Water Solubility | Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL). | | λmax | 548 nm | | Merck | 13,8097 | | BRN | 3795789 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | Biological Applications | Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides | | InChIKey | INCIMLINXXICKS-UHFFFAOYSA-M | | CAS DataBase Reference | 92-32-0 | | EPA Substance Registry System | Pyronine Y (92-32-0) |
| | PYRONIN Y Usage And Synthesis |
| Chemical Properties | green crystalline solid | | Uses | Bacterial and biological stain. | | Uses | Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections. | | Uses | Pyronin Y has been used:
- in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
- as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
- staining of CD34+ cells
| | Definition | ChEBI: An organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna
Pappenheim method. | | General Description | Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G. | | Biochem/physiol Actions | Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids. | | storage | Room temperature | | Purification Methods | Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.] |
| | PYRONIN Y Preparation Products And Raw materials |
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