4',5'-DIBROMOFLUORESCEIN

4',5'-DIBROMOFLUORESCEIN Basic information
Product Name:4',5'-DIBROMOFLUORESCEIN
Synonyms:Dibromofluorescein (contains Mono-, Tri- and Tetra-);4′,5′-Dibromofluorescein,Solvent Red72;SOLVENT RED 72;4',5'-Dibromof;4',5'-DibroMofluorescein, ca. 95% dye content;4',5'-dibromo-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one;4’,5’-dibromo-fluorescei;4’,5’-dibromofluororescein
CAS:596-03-2
MF:C20H10Br2O5
MW:490.1
EINECS:209-876-0
Product Categories:
Mol File:596-03-2.mol
4',5'-DIBROMOFLUORESCEIN Structure
4',5'-DIBROMOFLUORESCEIN Chemical Properties
Melting point 270-273 °C(lit.)
Boiling point 633.7±55.0 °C(Predicted)
density 1.6743 (rough estimate)
vapor pressure 0Pa at 25℃
refractive index 1.5010 (estimate)
storage temp. Sealed in dry,Room Temperature
form powder to crystal
pka8.14±0.20(Predicted)
Colour Index 45370
color Light yellow to Brown
Water Solubility 153.49μg/L at 25℃
Merck 14,3022
BRN 57189
CAS DataBase Reference596-03-2(CAS DataBase Reference)
EPA Substance Registry SystemSpiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4',5'-dibromo-3',6'-dihydroxy- (596-03-2)
Safety Information
Hazard Codes Xi,T
Risk Statements 36-25-36/37/38
Safety Statements 22-24/25-45-36-26
RIDADR UN 2811 6.1/PG 3
WGK Germany 3
RTECS LM5200000
TSCA Yes
HS Code 32129000
MSDS Information
ProviderLanguage
SigmaAldrich English
ACROS English
ALFA English
4',5'-DIBROMOFLUORESCEIN Usage And Synthesis
Chemical Propertiesorange powder
Uses4'',5''-Dibromofluorescein is a stain with an absorption maximum of 450 nm. Dyes and metabolites, Environmental Testing
PreparationFluorescein (C.I. Acid Yellow 73, C.I. 45350) in the sodium hydroxide solution two bromination, and??made into a free acid form.
Safety ProfilePoison by ingestion and intravenous routes. An herbicide. When heated to decomposition it emits highly toxic fumes of NOx, CN-, and Br-. See also NITRILES.
Properties and Applicationsbrilliant yellow light yellow. Slightly soluble in water for orange, with weak yellow fluorescence, soluble in ethanol for orange, with green yellow fluorescence, soluble in acetone peachblossom, with yellow fluorescence, soluble in Tetrahydrofurfuryl alcohol. In concentrated sulfuric acid to red light yellow, dilution after yellow brown, with orange precipitation. Dye aqueous solution to join sodium hydroxide as red edge; In the glycerol and liquid paraffin in good dispersibility. Used in medicines and cosmetics coloring.
Purification MethodsCrystallise it from aqueous 30% EtOH. [Beilstein 19/6 V 462.]
4',5'-DIBROMOFLUORESCEIN Preparation Products And Raw materials
Raw materialsFluorescein Sodium
Preparation ProductsEOSIN B
5(6)-CARBOXYEOSIN DIACETATE N-HYDROXY-SUCCINIMIDE ESTER 4',5'-DIBROMOFLUORESCEIN 5-(AND-6)-CARBOXYEOSIN DIACETATE, SUCCINIMIDYL ESTER 5-Aminoeosin 2',4',5',7'-TETRABROMO-3,4,5,6-TETRACHLOROFLUORESCEIN AZUR C EOSINATE EOSIN DIACETATE Acid Red 87 2',4',5',7'-TETRABROMO FLUORESCEIN, BIS(TRIETHYL)AMMONIUM SALT TETRACHROME STAIN 2',4',5',7'-TETRABROMO-4,5,6,7-TETRAFLUOROFLUORESCEIN DIACETATE dipotassium 3,6-dichloro-2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate 5(6)-CARBOXYEOSIN-N-HYDROXYSUCCINIMIDE ESTER EOSIN 5-ISOTHIOCYANATE Acid Red 92 5(6)-CARBOXYEOSIN DIACETATE EOSIN-5-IODOACETAMIDE LABOTEST-BB LT00159706

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